TiO2 나노튜브의 직경변화가 Raw 264.7 대식세포의 염증반응에 미치는 영향
The diameter effect of TiO2 nanotube on inflammatory response in Raw 264.7 Macrophages
문경숙, 배지명, 오승한,
소속 상세정보
문경숙 ( Mon Kyoung-Sook ) - 원광대학교 치과대학 치과생체재료학교실
배지명 ( Bae Ji-Myung ) - 원광대학교 치과대학 치과생체재료학교실
오승한 ( Oh Seung-Han ) - 원광대학교 치과대학 치과생체재료학교실
KMID : 0362220130400010025
Abstract
The biological studies related to the diameter of TiO2 nanotube have mainly focused on the biocompatibility and functionality of cells. The purpose of this study was to evaluate the size effect of various TiO2 nanotube diameters on inflammatory responses such as the generation of iNOS, NO and interleukin-1β(IL-1β), and tumor necrosis factor-α(TNF-α) in LPS-stimulated Raw 264.7 macrophages. The adhesion and proliferation of macrophages cultured on 100 nm TiO2 nanotube were significantly higher than those cultured on Ti(P<0.05). The results of nitric oxide(NO) generation from macrophage indicated that the value of 30 and 100 nm TiO2 nanotube had the tendency of reduction and increment, respectively, compared to Ti control group(P>0.05). The mRNA expression of iNOS, IL-1β and TNF-α resulted that the IL-1β (the degree of inflammation response) values of 30 nm TiO2 nanotube was significantly lower than those of Ti(P<0.05). In additions, IL-1β values of 100 nm TiO2 nanotube was significantly higher than those of Ti(P<0.05) Thus, it was concluded that TiO2 nanotube did not have cytotoxicity effect on RAW 264.7 macrophage, but 100 nm TiO2 nanotube had the potential for accelerating inflammation response in Raw 264.7 macrophages. Further investigation is required to resolve detailed mechanisms relating to the morphology and nanostructure of TiO2 nanotube and inflammation response.
키워드
TiO2 nanotube; Macrophage; Nitric oxide; iNOS; IL-1β; TNF-α; Inflammatory response.
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